Marker:Article Title: In Vitro Infant Digestion of Whey Proteins Isolate–Lactose
Article Snippet: .. In this investigation, Precision Plus Protein TM Dual Xtra Prestained Protein Standards (2–250) kDa were used as a protein marker, and gels were stained with Coomassie blue G-250 (161-0786, Bio-Rad, Hercules, CA, USA) following the procedures of [ ], with slight modification. ..
Staining:Article Title: In Vitro Infant Digestion of Whey Proteins Isolate–Lactose
Article Snippet: .. In this investigation, Precision Plus Protein TM Dual Xtra Prestained Protein Standards (2–250) kDa were used as a protein marker, and gels were stained with Coomassie blue G-250 (161-0786, Bio-Rad, Hercules, CA, USA) following the procedures of [ ], with slight modification. ..
Modification:Article Title: In Vitro Infant Digestion of Whey Proteins Isolate–Lactose
Article Snippet: .. In this investigation, Precision Plus Protein TM Dual Xtra Prestained Protein Standards (2–250) kDa were used as a protein marker, and gels were stained with Coomassie blue G-250 (161-0786, Bio-Rad, Hercules, CA, USA) following the procedures of [ ], with slight modification. ..
Article Title: Perilipin 5 links mitochondrial uncoupled respiration in brown fat to healthy white fat remodeling and systemic glucose tolerance
Article Snippet: .. Tris-glycine-SDS 10× solution, Fisher Bioreagents Cat # BP-1341-4; Tris-glycine 10× solution, Fisher Bioreagents Cat # BP-1306-4; Tris-buffered saline, G Biosciences, Cat # R030; Tween-20, Acros Organics Cat # 23336-0010; methanol, Fisher Chemical Cat # A412SK; BSA, Fisher Scientific Cat # BP16001; free fatty acid BSA, Fitzgerald Cat # 30-AB79; HyCloneTM Dulbecco’s Modified Eagles Medium, GE Healthcare Life Sciences Cat # SH30022.01; QIAzol Lysis Reagent, Qiagen Cat # 79306; Criterion TM TGX TM 4–20%, Bio-Rad Cat # 5671094; Criterion TM TGX TM Any Kd, Bio-Rad Cat # 5671124; nitrocellulose membrane, Bio-Rad Cat # 1620112; Power Sybr Green, Applied Biosystems Cat # 4367659; Precision Plus Protein TM Dual Xtra Prestained Protein Standards, Bio-Rad Cat # 1610377; Dulbecco’s phosphate-buffered saline, Sigma-Aldrich Cat # D8662; Infinity TM triglycerides liquid stable reagent, Thermo Scientific Cat # TR22421; Multi calibrator lipids, Wako Cat # 464-01601; HR series NEFA-HR, Wako Cat # 999-34691; NEFA standard solution, Wako Cat # 276-76491; free glycerol reagent, Sigma-Aldrich Cat # F6428; Glycerol Standard solution, Sigma-Aldrich Cat # G7793; Pierce 660 nM Protein Assay Reagent, Thermo Scientific Cat # 22660; 3a70B Complete Counting cocktail, RPI Cat # 111154; Solvable TM , Perkin Elmer Cat # 6NE9100; paraformaldehyde, Sigma-Aldrich Cat # P6148; sodium pyruvate, Sigma-Aldrich Cat # P5280; l -(−)-malic acid, Sigma-Aldrich Cat # M1000; GDP sodium salt, Sigma-Aldrich Cat # G7127; d -(+)-glucose, Sigma-Aldrich Cat # G7528; d -sucrose, Fisher Bioreagents Cat # BP220-1; d -mannitol, Sigma-Aldrich Cat # M4125; HEPES 1 M solution, Sigma-Aldrich Cat # H0887; EGTA, MP Biomedicals Cat # 195174; potassium phosphate monobasic (KH 2 PO 4 ), Sigma-Aldrich Cat # P5655; magnesium chloride (MgCl 2 ), Sigma-Aldrich Cat # M8266; Tris base, Fisher Bioreagents Cat # BP152-1; sodium chloride (NaCl 2 ), Fisher Scientific Cat # S671-3; sodium deoxycholate, Sigma-Aldrich Cat # 30970; Tris-HCl 1 M solution, Fisher Bioreagents Cat # BP1757; glycerol, Acros Organics Cat # 327255000; orange G, Sigma-Aldrich Cat # O3756; sodium hydroxide (NaOH), Fisher Scientific Cat # S318-1; sodium dodecyl sulfate (SDS), Invitrogen Cat # 15-525-017; chloroform, Sigma-Aldrich, Cat # C24322; mercaptoethanol, Fisher Scientific Cat # 03446I-100; glacial acetic acid, Fisher Bioreagents Cat # BP2401. .. For statistical analysis, we used GraphPad Prism version 7.00 for MacOS, GraphPad Software, La Jolla California USA, www.graphpad.com .
Molecular Weight:Article Title: γ-Conglutin Immunoreactivity Is Differently Affected by Thermal Treatment and Gastrointestinal Digestion in Lupine Species
Article Snippet: Gel image was collected using a white tray and processed with Image Lab 5.2.1 software (Gel Doc TM EZ Imager, Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. Precision Plus Protein TM Dual Xtra Prestained Protein Standards (10–250 kDa, Bio-Rad Laboratories, Inc., Hercules, CA, USA) was used as protein molecular weight reference. ..
Article Title: Transient receptor potential melastatin 6 and transient receptor potential melastatin 6/7 antagonists suppress colon adenocarcinoma HT-29 cells
Article Snippet: .. Precision Plus Protein TM Dual Xtra Prestained Protein Standards (Cat. No. 1610377; Bio-Rad Laboratories, Hercules, CA, United States) were used as molecular weight markers. .. The membranes were probed with primary antibodies targeting TRPM6 (PA5-77326; Thermo Fisher Scientific, Waltham, MA, United States), TRPM7 (ab729; Abcam, Cambridgeshire, United Kingdom), matrix metalloproteinase-9 (MMP-9: Ab228402 ; Abcam, Cambridgeshire, United Kingdom), cyclooxygenase-2 (COX-2: Ab179800 ; Abcam, Cambridgeshire, United Kingdom), Nanog (ab317506; Abcam, Cambridgeshire, United Kingdom), Na + /K + -ATPase (ab76020; Abcam, Cambridgeshire, United Kingdom), and β-actin (ab8226; Abcam, Cambridgeshire, United Kingdom), followed by horseradish peroxidase-conjugated secondary antibodies.
Saline:Article Title: Perilipin 5 links mitochondrial uncoupled respiration in brown fat to healthy white fat remodeling and systemic glucose tolerance
Article Snippet: .. Tris-glycine-SDS 10× solution, Fisher Bioreagents Cat # BP-1341-4; Tris-glycine 10× solution, Fisher Bioreagents Cat # BP-1306-4; Tris-buffered saline, G Biosciences, Cat # R030; Tween-20, Acros Organics Cat # 23336-0010; methanol, Fisher Chemical Cat # A412SK; BSA, Fisher Scientific Cat # BP16001; free fatty acid BSA, Fitzgerald Cat # 30-AB79; HyCloneTM Dulbecco’s Modified Eagles Medium, GE Healthcare Life Sciences Cat # SH30022.01; QIAzol Lysis Reagent, Qiagen Cat # 79306; Criterion TM TGX TM 4–20%, Bio-Rad Cat # 5671094; Criterion TM TGX TM Any Kd, Bio-Rad Cat # 5671124; nitrocellulose membrane, Bio-Rad Cat # 1620112; Power Sybr Green, Applied Biosystems Cat # 4367659; Precision Plus Protein TM Dual Xtra Prestained Protein Standards, Bio-Rad Cat # 1610377; Dulbecco’s phosphate-buffered saline, Sigma-Aldrich Cat # D8662; Infinity TM triglycerides liquid stable reagent, Thermo Scientific Cat # TR22421; Multi calibrator lipids, Wako Cat # 464-01601; HR series NEFA-HR, Wako Cat # 999-34691; NEFA standard solution, Wako Cat # 276-76491; free glycerol reagent, Sigma-Aldrich Cat # F6428; Glycerol Standard solution, Sigma-Aldrich Cat # G7793; Pierce 660 nM Protein Assay Reagent, Thermo Scientific Cat # 22660; 3a70B Complete Counting cocktail, RPI Cat # 111154; Solvable TM , Perkin Elmer Cat # 6NE9100; paraformaldehyde, Sigma-Aldrich Cat # P6148; sodium pyruvate, Sigma-Aldrich Cat # P5280; l -(−)-malic acid, Sigma-Aldrich Cat # M1000; GDP sodium salt, Sigma-Aldrich Cat # G7127; d -(+)-glucose, Sigma-Aldrich Cat # G7528; d -sucrose, Fisher Bioreagents Cat # BP220-1; d -mannitol, Sigma-Aldrich Cat # M4125; HEPES 1 M solution, Sigma-Aldrich Cat # H0887; EGTA, MP Biomedicals Cat # 195174; potassium phosphate monobasic (KH 2 PO 4 ), Sigma-Aldrich Cat # P5655; magnesium chloride (MgCl 2 ), Sigma-Aldrich Cat # M8266; Tris base, Fisher Bioreagents Cat # BP152-1; sodium chloride (NaCl 2 ), Fisher Scientific Cat # S671-3; sodium deoxycholate, Sigma-Aldrich Cat # 30970; Tris-HCl 1 M solution, Fisher Bioreagents Cat # BP1757; glycerol, Acros Organics Cat # 327255000; orange G, Sigma-Aldrich Cat # O3756; sodium hydroxide (NaOH), Fisher Scientific Cat # S318-1; sodium dodecyl sulfate (SDS), Invitrogen Cat # 15-525-017; chloroform, Sigma-Aldrich, Cat # C24322; mercaptoethanol, Fisher Scientific Cat # 03446I-100; glacial acetic acid, Fisher Bioreagents Cat # BP2401. .. For statistical analysis, we used GraphPad Prism version 7.00 for MacOS, GraphPad Software, La Jolla California USA, www.graphpad.com .
Lysis:Article Title: Perilipin 5 links mitochondrial uncoupled respiration in brown fat to healthy white fat remodeling and systemic glucose tolerance
Article Snippet: .. Tris-glycine-SDS 10× solution, Fisher Bioreagents Cat # BP-1341-4; Tris-glycine 10× solution, Fisher Bioreagents Cat # BP-1306-4; Tris-buffered saline, G Biosciences, Cat # R030; Tween-20, Acros Organics Cat # 23336-0010; methanol, Fisher Chemical Cat # A412SK; BSA, Fisher Scientific Cat # BP16001; free fatty acid BSA, Fitzgerald Cat # 30-AB79; HyCloneTM Dulbecco’s Modified Eagles Medium, GE Healthcare Life Sciences Cat # SH30022.01; QIAzol Lysis Reagent, Qiagen Cat # 79306; Criterion TM TGX TM 4–20%, Bio-Rad Cat # 5671094; Criterion TM TGX TM Any Kd, Bio-Rad Cat # 5671124; nitrocellulose membrane, Bio-Rad Cat # 1620112; Power Sybr Green, Applied Biosystems Cat # 4367659; Precision Plus Protein TM Dual Xtra Prestained Protein Standards, Bio-Rad Cat # 1610377; Dulbecco’s phosphate-buffered saline, Sigma-Aldrich Cat # D8662; Infinity TM triglycerides liquid stable reagent, Thermo Scientific Cat # TR22421; Multi calibrator lipids, Wako Cat # 464-01601; HR series NEFA-HR, Wako Cat # 999-34691; NEFA standard solution, Wako Cat # 276-76491; free glycerol reagent, Sigma-Aldrich Cat # F6428; Glycerol Standard solution, Sigma-Aldrich Cat # G7793; Pierce 660 nM Protein Assay Reagent, Thermo Scientific Cat # 22660; 3a70B Complete Counting cocktail, RPI Cat # 111154; Solvable TM , Perkin Elmer Cat # 6NE9100; paraformaldehyde, Sigma-Aldrich Cat # P6148; sodium pyruvate, Sigma-Aldrich Cat # P5280; l -(−)-malic acid, Sigma-Aldrich Cat # M1000; GDP sodium salt, Sigma-Aldrich Cat # G7127; d -(+)-glucose, Sigma-Aldrich Cat # G7528; d -sucrose, Fisher Bioreagents Cat # BP220-1; d -mannitol, Sigma-Aldrich Cat # M4125; HEPES 1 M solution, Sigma-Aldrich Cat # H0887; EGTA, MP Biomedicals Cat # 195174; potassium phosphate monobasic (KH 2 PO 4 ), Sigma-Aldrich Cat # P5655; magnesium chloride (MgCl 2 ), Sigma-Aldrich Cat # M8266; Tris base, Fisher Bioreagents Cat # BP152-1; sodium chloride (NaCl 2 ), Fisher Scientific Cat # S671-3; sodium deoxycholate, Sigma-Aldrich Cat # 30970; Tris-HCl 1 M solution, Fisher Bioreagents Cat # BP1757; glycerol, Acros Organics Cat # 327255000; orange G, Sigma-Aldrich Cat # O3756; sodium hydroxide (NaOH), Fisher Scientific Cat # S318-1; sodium dodecyl sulfate (SDS), Invitrogen Cat # 15-525-017; chloroform, Sigma-Aldrich, Cat # C24322; mercaptoethanol, Fisher Scientific Cat # 03446I-100; glacial acetic acid, Fisher Bioreagents Cat # BP2401. .. For statistical analysis, we used GraphPad Prism version 7.00 for MacOS, GraphPad Software, La Jolla California USA, www.graphpad.com .
Article Title: DLin-MC3-Containing mRNA Lipid Nanoparticles Induce an Antibody Th2-Biased Immune Response Polarization in a Delivery Route-Dependent Manner in Mice
Article Snippet: Absolute ethanol was purchased from Carlo Erba Reagents (Peypin, France). .. RIPA lysis buffer and 100× Halt Protease inhibitor cocktail were procured from Thermo Fisher Scientific (Waltham, MA, USA). β-Mercaptoethanol, 4× Laemmli sample buffer, and Precision Plus Protein TM Dual Xtra Prestained Protein Standards were purchased from BioRad (Marnes-la-Coquette, France). .. A GenVoy-ILM TM nonviral lipid nanoparticle kit was donated by Precision Nanosystems Inc (Vancouver, BC, Canada). mRNAs encoding for firefly luciferase (FLuc) and p55Gag HIV polyprotein were purchased from Trilink BioTechnologies (San Diego, CA, USA).
Membrane:Article Title: Perilipin 5 links mitochondrial uncoupled respiration in brown fat to healthy white fat remodeling and systemic glucose tolerance
Article Snippet: .. Tris-glycine-SDS 10× solution, Fisher Bioreagents Cat # BP-1341-4; Tris-glycine 10× solution, Fisher Bioreagents Cat # BP-1306-4; Tris-buffered saline, G Biosciences, Cat # R030; Tween-20, Acros Organics Cat # 23336-0010; methanol, Fisher Chemical Cat # A412SK; BSA, Fisher Scientific Cat # BP16001; free fatty acid BSA, Fitzgerald Cat # 30-AB79; HyCloneTM Dulbecco’s Modified Eagles Medium, GE Healthcare Life Sciences Cat # SH30022.01; QIAzol Lysis Reagent, Qiagen Cat # 79306; Criterion TM TGX TM 4–20%, Bio-Rad Cat # 5671094; Criterion TM TGX TM Any Kd, Bio-Rad Cat # 5671124; nitrocellulose membrane, Bio-Rad Cat # 1620112; Power Sybr Green, Applied Biosystems Cat # 4367659; Precision Plus Protein TM Dual Xtra Prestained Protein Standards, Bio-Rad Cat # 1610377; Dulbecco’s phosphate-buffered saline, Sigma-Aldrich Cat # D8662; Infinity TM triglycerides liquid stable reagent, Thermo Scientific Cat # TR22421; Multi calibrator lipids, Wako Cat # 464-01601; HR series NEFA-HR, Wako Cat # 999-34691; NEFA standard solution, Wako Cat # 276-76491; free glycerol reagent, Sigma-Aldrich Cat # F6428; Glycerol Standard solution, Sigma-Aldrich Cat # G7793; Pierce 660 nM Protein Assay Reagent, Thermo Scientific Cat # 22660; 3a70B Complete Counting cocktail, RPI Cat # 111154; Solvable TM , Perkin Elmer Cat # 6NE9100; paraformaldehyde, Sigma-Aldrich Cat # P6148; sodium pyruvate, Sigma-Aldrich Cat # P5280; l -(−)-malic acid, Sigma-Aldrich Cat # M1000; GDP sodium salt, Sigma-Aldrich Cat # G7127; d -(+)-glucose, Sigma-Aldrich Cat # G7528; d -sucrose, Fisher Bioreagents Cat # BP220-1; d -mannitol, Sigma-Aldrich Cat # M4125; HEPES 1 M solution, Sigma-Aldrich Cat # H0887; EGTA, MP Biomedicals Cat # 195174; potassium phosphate monobasic (KH 2 PO 4 ), Sigma-Aldrich Cat # P5655; magnesium chloride (MgCl 2 ), Sigma-Aldrich Cat # M8266; Tris base, Fisher Bioreagents Cat # BP152-1; sodium chloride (NaCl 2 ), Fisher Scientific Cat # S671-3; sodium deoxycholate, Sigma-Aldrich Cat # 30970; Tris-HCl 1 M solution, Fisher Bioreagents Cat # BP1757; glycerol, Acros Organics Cat # 327255000; orange G, Sigma-Aldrich Cat # O3756; sodium hydroxide (NaOH), Fisher Scientific Cat # S318-1; sodium dodecyl sulfate (SDS), Invitrogen Cat # 15-525-017; chloroform, Sigma-Aldrich, Cat # C24322; mercaptoethanol, Fisher Scientific Cat # 03446I-100; glacial acetic acid, Fisher Bioreagents Cat # BP2401. .. For statistical analysis, we used GraphPad Prism version 7.00 for MacOS, GraphPad Software, La Jolla California USA, www.graphpad.com .
SYBR Green Assay:Article Title: Perilipin 5 links mitochondrial uncoupled respiration in brown fat to healthy white fat remodeling and systemic glucose tolerance
Article Snippet: .. Tris-glycine-SDS 10× solution, Fisher Bioreagents Cat # BP-1341-4; Tris-glycine 10× solution, Fisher Bioreagents Cat # BP-1306-4; Tris-buffered saline, G Biosciences, Cat # R030; Tween-20, Acros Organics Cat # 23336-0010; methanol, Fisher Chemical Cat # A412SK; BSA, Fisher Scientific Cat # BP16001; free fatty acid BSA, Fitzgerald Cat # 30-AB79; HyCloneTM Dulbecco’s Modified Eagles Medium, GE Healthcare Life Sciences Cat # SH30022.01; QIAzol Lysis Reagent, Qiagen Cat # 79306; Criterion TM TGX TM 4–20%, Bio-Rad Cat # 5671094; Criterion TM TGX TM Any Kd, Bio-Rad Cat # 5671124; nitrocellulose membrane, Bio-Rad Cat # 1620112; Power Sybr Green, Applied Biosystems Cat # 4367659; Precision Plus Protein TM Dual Xtra Prestained Protein Standards, Bio-Rad Cat # 1610377; Dulbecco’s phosphate-buffered saline, Sigma-Aldrich Cat # D8662; Infinity TM triglycerides liquid stable reagent, Thermo Scientific Cat # TR22421; Multi calibrator lipids, Wako Cat # 464-01601; HR series NEFA-HR, Wako Cat # 999-34691; NEFA standard solution, Wako Cat # 276-76491; free glycerol reagent, Sigma-Aldrich Cat # F6428; Glycerol Standard solution, Sigma-Aldrich Cat # G7793; Pierce 660 nM Protein Assay Reagent, Thermo Scientific Cat # 22660; 3a70B Complete Counting cocktail, RPI Cat # 111154; Solvable TM , Perkin Elmer Cat # 6NE9100; paraformaldehyde, Sigma-Aldrich Cat # P6148; sodium pyruvate, Sigma-Aldrich Cat # P5280; l -(−)-malic acid, Sigma-Aldrich Cat # M1000; GDP sodium salt, Sigma-Aldrich Cat # G7127; d -(+)-glucose, Sigma-Aldrich Cat # G7528; d -sucrose, Fisher Bioreagents Cat # BP220-1; d -mannitol, Sigma-Aldrich Cat # M4125; HEPES 1 M solution, Sigma-Aldrich Cat # H0887; EGTA, MP Biomedicals Cat # 195174; potassium phosphate monobasic (KH 2 PO 4 ), Sigma-Aldrich Cat # P5655; magnesium chloride (MgCl 2 ), Sigma-Aldrich Cat # M8266; Tris base, Fisher Bioreagents Cat # BP152-1; sodium chloride (NaCl 2 ), Fisher Scientific Cat # S671-3; sodium deoxycholate, Sigma-Aldrich Cat # 30970; Tris-HCl 1 M solution, Fisher Bioreagents Cat # BP1757; glycerol, Acros Organics Cat # 327255000; orange G, Sigma-Aldrich Cat # O3756; sodium hydroxide (NaOH), Fisher Scientific Cat # S318-1; sodium dodecyl sulfate (SDS), Invitrogen Cat # 15-525-017; chloroform, Sigma-Aldrich, Cat # C24322; mercaptoethanol, Fisher Scientific Cat # 03446I-100; glacial acetic acid, Fisher Bioreagents Cat # BP2401. .. For statistical analysis, we used GraphPad Prism version 7.00 for MacOS, GraphPad Software, La Jolla California USA, www.graphpad.com .
Protease Inhibitor:Article Title: DLin-MC3-Containing mRNA Lipid Nanoparticles Induce an Antibody Th2-Biased Immune Response Polarization in a Delivery Route-Dependent Manner in Mice
Article Snippet: Absolute ethanol was purchased from Carlo Erba Reagents (Peypin, France). .. RIPA lysis buffer and 100× Halt Protease inhibitor cocktail were procured from Thermo Fisher Scientific (Waltham, MA, USA). β-Mercaptoethanol, 4× Laemmli sample buffer, and Precision Plus Protein TM Dual Xtra Prestained Protein Standards were purchased from BioRad (Marnes-la-Coquette, France). .. A GenVoy-ILM TM nonviral lipid nanoparticle kit was donated by Precision Nanosystems Inc (Vancouver, BC, Canada). mRNAs encoding for firefly luciferase (FLuc) and p55Gag HIV polyprotein were purchased from Trilink BioTechnologies (San Diego, CA, USA).
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